Mango is considered by many the king of fruits and represents an important nutraceutical and antioxidant source. It possesses high nutritional value with a great diversity in colour, size, aroma, and flavour, and some allergens. Mango production is one of the ten major fruits produced worldwide. In Mexico in the last year (2024) the production of mango was above 2 million fruits which represented earnings above 13 million pesos, making Mexico the fifth world producer (SIAP, 2024). However, despite its economic importance, there is still a lack of knowledge about the molecular mechanisms underlying the changes in mango through the ripening stage at the postharvest period.This thesis aimed to identify molecular mechanisms related to postharvest life in mango cultivar Tommy Atkin peel at two different times during the postharvest period (the day of harvesting and six days after harvesting). To achieve this, we established and tested various proteomic protocols. First, we explore different extraction techniques (phenol-based and methanol-chloroform-based) and peptide fractionation method (strong cation exchange and high-pH reversed phase). Second, we carried out quantitative protein approaches including Label-Free Quantitative (LFQ) and Tandem Mass Tag SPS-MS3 and raw data was analysed with various search engines such as SEQUEST, AMANDA, COMET, and Byonic. Our analysis exhibited a great complementation of each strategy for protein identification. Besides, LFQ and Byonic search engines allowed to determine the presence of N-glycoproteins without previous enrichment. In a third stage of our study we tested, the performance of artificial intelligence-driven search engines INFERYS and CHIMERYS over a conventional search engine (SEQUEST). We supported our findings with metabolomics and gene expression techniques. By doing so, we corroborate our proteomic data.This work represents the largest proteomic comparison of mango peels during the postharvest period made so far. We found evidence that suggests a fine-tuned crosstalk of plant growth regulators whose output regulates the flavonoid biosynthesis and redox balance of mango peels in the postharvest period. Therefore, we contributed to a better understanding of the molecular changes behind the ripening process of mango in the postharvest period, providing essential information about mango peels.

Consulta esta tesis en la clasificación: TDD20250002

Forma sugerida para citar

• Bautista Valle, Mirna Verónica. (2025). Proteomic studies in fruits of the Tommy Atkins mango cultivar (Mangifera indica L.) at postharvest stages [Tesis de Doctorado]. Instituto de Ecología, A.C.